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Image Search Results
Journal: BMC Gastroenterology
Article Title: Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the small bowel in patients with severe irritable bowel syndrome
doi: 10.1186/1471-230x-10-19
Figure Lengend Snippet: Figure 1 Fluorescent microscope images of small bowel preparations from patients with IBS A. Chlamydia LPS in EEC-like cells with apical nuclei and strong basal immunofluorescence (arrows). (Monoclonal FITC-conjugated antibody with Evans blue; original magnification × 63). B. Chlamydia LPS in a few cells within the epithelium (thick arrows) and l. propria (thin arrows). (Monoclonal FITC-conjugated antibody with Evans blue; original magnification × 63). C. Chlamydia trachomatis MOMP-positive immunofluorescence within 2 EEC-like cells (arrows) and 4 cells within l. propria (arrowheads). (Mouse MOMP-antibody and FITC-conjugated rabbit anti-mouse antibody; original magnification × 63). Hoechst (DAPI conjugated) for nuclear staining. D-F. Immunostainings for (D) Chlamydia LPS (FITC, green), (E) chromogranin A (Alexia 568, red), and (F) merged showing co-localisation of chromogranin A and Chlamydia LPS in enteroendocrine cells and Chlamydia LPS in l. propria. G-I. Immunostainings for (G) Chlamydia LPS (FITC, green,) (H) CD68 (Alexia 350. blue), and (I) merged showing co-localisation of CD68 and Chlamydia LPS in macrophages (arrows). Three enteroendocrine cells are also positive for Chlamydia LPS (arrowheads).
Article Snippet: Immunofluorescence was performed using a genus-specific
Techniques: Microscopy, Immunofluorescence, Staining
Journal: Microorganisms
Article Title: Virulence of Francisella tularensis Subspecies holarctica Biovar japonica and Phenotypic Change during Serial Passages on Artificial Media
doi: 10.3390/microorganisms8121881
Figure Lengend Snippet: Antigenic change in lipopolysaccharide (LPS) of KU-1 variants passaged on Eugon chocolate agar in Western blots. Purified LPSs of KU-1 original and the variants passaged on Eugon chocolate agar 10, 20, and 80 times (EP10, EP20, and EP80, respectively) and LVS (lanes 1, 2, 3, 4, and 5, respectively) were detected using MAbs M15C6, M13A13, and FB11 [sheets ( A ), ( B ), and ( C ), respectively]. M: molecular weight markers (Bio-Rad) indicate 75, 50, 37, 25, 20, 15, and 10 (arrow) kDa from the top.
Article Snippet:
Techniques: Chocolate, Western Blot, Purification, Molecular Weight